(080) Exploring the Effects of Actinidin as a Novel Collagenase on Human Peyronie’s Disease Cells

Abstract Introduction Peyronie’s Disease (PD) is a connective tissue disorder of the penile tunica albuginea that affects approximately 10% men. The plaques hallmark PD contain excessive amounts collagen and can result in curvature, painful erections, and/or erectile dysfunction. Intralesional Collagenase Clostridium histolyticum injection for are no longer available Canada, making treatment options limited. Hayward kiwi fruit rich natural actinidin, soluble enzyme has unique ability to hydrolyze various types fibrinogen. Actinidin considered collagenase alternative; however, there have been studies evaluating application actinidin on vitro cellular models. Objective To determine effectiveness at reducing component 2D human cell model. Secondly, cytotoxicity kiwifruit extract cells. Methods Human tissues obtained during prosthesis implantation were isolated fibroblast cells cultured using complete media. plated 96-well plate treated with one 4 groups: media, saline, low high concentration (0.5 mg/ml 37.5 mg/ml). treatments prepared by dissolving freeze-dried powder saline then filtered; was further diluted saline. Cells incubated 37°C 24 hours extracted measured quantification assay kit fluorescent microplate reader. measure cytotoxicity, stained nucleus actin filaments DAPI Phalloidin-conjugated FITC, respectively. A twelve-hour timelapse capturing images FITC fluorescein carried through confocal microscope. total area each well ImageJ. Actin signals normalized signals, all values analyzed Python Prism10. Results higher significantly reduced fibroblasts compared media (P < 0.05). had signal than other groups 0.0001). This ratio allows us observe compromised plasma membranes. did not any significant difference from groups. increased level indicated may mechanisms disturb membrane. Conclusions Our preliminary study suggests break down extracellular content compromising Further underway investigate 3D models optimal reduce composition potential be novel option patients suffering PD. Disclosure Yes, this sponsored industry/sponsor: KiwiEnzyme.com Ltd Clarification No industry support design or execution